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The sections were then incubated in blocking solution (PBS supplemented with 2% Normal Goat Serum, 1% BSA, 1% Triton-X, .05% Tween-20, and .05% sodium azide) at room temperature for 1 h and incubated at 4C overnight with the following primary antibodies

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In spite of 40 years of research the exact function of this protein is more complex than ever, but it has been found that these intracellular dimeric proteins, play a major role in the intracellular transport of endogenous compounds, metabolizes various electrophilic xenobiotics, ligand transport and thus protects cells against toxic effects [31], [87], [85]

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