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After 20 days, cells were expanded, and inactivation of the desire gene confirmed by western blot using GCLM (Atlas antibodies, #HPA023696, 1:500) or ADH5 (1:500) antibodies 9

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After tipping off the supernatant, plates were washed, blotted on paper and blocked with PBS 1% BSA for 30 min at RT

Drinking enough fluids and replacing electrolytes can help support your daily routine, especially if you are training, sweating, or eating less than usual

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