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Features were ranked according to the chi-square scores between each individual feature and the target Experimental Group (Control, undSOD1, mutSOD1)

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MEGAN Ultimate version 6.10.2 52 was used to associate metagenome sequences with both taxa and Kyoto Encyclopaedia of Genes and Genomes (KEGG) functional orthologs and modules 53

In humans, follicular fluid vitamin D levels correlate with embryo quality, supporting its use in vitamin D-deficient patients undergoing ICSI ( Dynamic biomarkers, such as mitochondrial membrane potential or redox balance (GSH/GSSG ratio), could guide dose adjustments