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Yeast one-hybrid (Y1H) analysis The 1456 bp upstream sequence from +1 site of AtNRAMP3, 1500 bp upstream sequence from +1 site of both AtNRAMP4 and AtPIC1 genes were amplified by PCR and subsequently cloned into the KpnI and SacI RE sites of the pAbAi vector (Takara)

In CD38-deficient animals, NAD+ levels, mitochondrial respiratory rates, and metabolic activities remained constant with age (7)

The authors have declared that no competing interest exists

Furthermore, we offer insights into circadian rhythmregulating therapies, which may contribute to the development of novel strategies for the prevention and treatment of circadian rhythmrelated diseases

A high level of Zonulin (a marker we can measure) is more likely to be associated with greater intestinal permeability