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(4) Oocytes were mechanically released from ovaries under M2 medium supplemented with 3 mg/ml bovine serum albumin (BSA) in the presence of the meiotic arrest inhibitor 3-isobutyl-1-methylxanthine (IBMX) (Sigma-Aldrich, Australia) at 50 M

Cells were plated on glass chamber slides, fixed and stained with indicated antibodies

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