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The cell lysate was centrifuged (15,000 g for 30 min) at 4 C to remove the cell debris, and the resulting supernatant was loaded onto a Fast Flow Ni 2+ -agarose column (GE Healthcare, USA), which was pre-equilibrated with buffer A

doi: 10.1126/science.276.5316.1239, 56 KivelsonM

Functional analysis of the human perivascular subarachnoid space

In accordance with another embodiment, peptides having the structural formules hereinabove described, and wherein at least one of amino acid residues can be omitted and at least one of the remaining amino acid residues can be substituted as indicated in table I, are transformed in cyclic form by the formation of a new bond CO-NH between the first and the last amino acid residues in the molecule

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Liraglutide (Saxenda) Saxenda was the first GLP-1 receptor agonist licensed for weight management in the UK