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10.1155/2022/4560607 59 DevkotaH

The reaction mixture consisted of 25 L of the extract, 75 L of tricine-KOH buffer (pH 9.0), 6 L of 12 mmol L 1 EDTA-2Na, 2.5 L of 0.3 mmol L 1 phenazine ethosulfate, 50 L of 1.8 mmol L 1 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl-2H-tetrazolium bromide (MTT), 9 L of 1.5 M ethanol, and 100 L of H 2 O

in vitro NAFLD model using palmitate-treated HepG2 cells, which mimic lipid overload and metabolic stress typical of early disease

In another instance, overexpression of the Cu/Zn-SOD gene in Nicotiana tabacum plants was done to improve oxidative stress resistance, while tolerance to methyl viologen and pure cercosporin was achieved in sugar beet by transferring a Lycopersicon esculentum SOD gene (Tertivanidis et al., 2004

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Despite some earlier in vitro studies, the first evidence in intact animals suggesting MT's cardioprotective role in myocardia I/R injury was shown in a cardiac-specific MT-overexpressing transgenic mouse model 87