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Future research should consider incorporating biochemical verification methods to enhance the validity and reliability of smoking abstinence assessments [113]

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Journal of the National Cancer Institute , 96(11), 853861

The reverse transcription reaction was then carried out using High Capacity RNA to cDNA Kit (Invitrogen, USA) and followed by qPCR performed with specific TaqMan gene expression assays for FLG , LOR , IVL , KLK7 , TGM1 , and RPL13A genes (all ThermoFisher Scientific, USA) according to the manufacturers recommendations